Journal: Cell Death & Disease

Article Title: MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4

doi: 10.1038/s41419-020-02789-z

Figure Lengend Snippet: a Expression level of miR-3622a-3p was detected in CRC cell lines and NCM460 cell line by qRT-PCR. b Expression of miR-3622a-3p was increased in DLD-1 by miR-3622a-3p mimics transfection. c MiR-3622a-3p expression level was reduced in HCT116 by miR-3622a-3p inhibitor transfection. d , e The effect of miR-3622a-3p on proliferation of CRC cells was evaluated by CCK-8 cell proliferation assay. f , g Colony forming ability of CRC cells was negatively corelated with miR-3622a-3p expression level. h , i The results of EDU assay suggested overexpression of miR-3622a-3p suppressed proliferation of CRC cells while knockdown of miR-3622a-3p promoted CRC cell proliferation. All data are from three independent experiments and are presented as the means ± SD (* p < 0.05, ** p < 0.01).

Article Snippet: Human normal colon epithelial cell line NCM460 was obtained from American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Quantitative RT-PCR, Transfection, CCK-8 Assay, Proliferation Assay, EdU Assay, Over Expression, Knockdown

Journal: Neural Regeneration Research

Article Title: Elucidation of the mechanism by which manganese-ferric Prussian blue nanozymes alleviate ischemic stroke damage in a mouse model

doi: 10.4103/nrr.nrr-d-24-00837

Figure Lengend Snippet: Figure 6 ｜Therapeutic mechanisms of MnPB nanozymes and SCFAs in MCAO model. (A) Illustration of the concentration-dependent cellular uptake of MnPB nanozymes, highlighting the biocompatibility and internalization efficiency in NCM460 cells. Scale bar: 50 μm. (B) Assessment of the toxic effects of MnPB nanozymes on NCM460 cells, demonstrating safety within a concentration range of 0–50 μg/mL (n = 5). (C) Assessment of the toxic effects of MnPB nanozymes on BV-2 cells, demonstrating safety within a concentration range of 0–50 μg/mL (n = 5) (D) Examination of the toxic impact of H2O2 on BV-2 cells, establishing a baseline for oxidative stress-induced injury (n = 5). (E) Evaluation of the protective effects of different concentrations of MnPB nanozymes and SCFAs on cell survival in an in vitro oxidative stress injury model induced by H2O2 (n = 5). (F–H) Determination of the cytotoxicity of different concentrations of sodium acetate (NaA), sodium propionate (NaP), and sodium butyrate (NaB) on BV-2 cells after a 12-hour treatment period using the CCK-8 kit (n = 5). (I) Assessment of the combined toxicity of NaA, NaP, NaB, and SCFAs mixtures on BV-2 cells after a 12-hour exposure, confirming the safety of the selected concentration ratios (n = 5). (J) Utilization of the DCFH-DA assay to measure the reactive oxygen species (ROS) scavenging effect of varying concentrations of SCFAs. (K) Quantitative analysis of ROS spectral intensity, providing insights into the antioxidant capacity of SCFAs (n = 3). (L) Flow cytometry results depicting the apoptotic effects in BV-2 cells under oxidative stress. (M) Statistical analysis of apoptosis rates, revealing the protective role of SCFAs against cell death (n = 3). (N) Analysis of LPS-induced inflammation in BV-2 cells and the subsequent impact of SCFAs supplementation on the expression of TLR4, IKKα, and p65, as detected by immunoblotting. (O) Presentation of protein expression level statistics, indicating the modulatory effects of SCFAs on the TLR4/NF-κB pathway (n = 3). (P) Detection of TLR4, IKKα, and pp65 expression in BV-2 cells following blockade of the TLR4 pathway, further elucidating the mechanism of action (n = 3). (Q) Quantitative analysis of protein expression levels, reinforcing the role of TLR4/NF-κB signaling in the observed effects (n = 3). (R) Immunoblotting detection of TLR4, IKKα, and pp65 expression in brain tissues, providing in vivo evidence of the pathway’s involvement. (S) Quantitative analysis of protein expression density relative to the control group, underscoring the significance of TLR4/NF-κB modulation in the therapeutic effects (n = 3). Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, vs. Control group; #P < 0.05, ##P < 0.01, ###P < 0.001, vs. MCAO group (one- way analysis of variance followed by Tukey’s post hoc test). CCK-8: Cell counting kit-8; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IKKα: inhibitory kappa B kinase α; MCAO: middle cerebral artery occlusion; MnPB: manganese-iron Prussian blue; NF-κB: nuclear factor κB; RBITC: Rhodamine B isothiocyanate; SCFA: short-chain fatty acid; TLR4: Toll-like receptor 4.

Article Snippet: Cell culture and in vitro experimental design The human normal colonic epithelial cell line NCM460 (American Type Culture Collection, Manassas, VA, USA, CRL-1807, RRID:CVCL_2872) and BV-2 microglial cells (American Type Culture Collection, CRL-2467, RRID:CVCL_5744) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Carlsbad, CA, USA, Cat# 11965092) containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA, Cat# A5669701) and 1% of an antibiotic mixture containing penicillin/streptomycin (Gibco, Cat# 15140122).

Techniques: Concentration Assay, In Vitro, CCK-8 Assay, DCFH-DA Assay, Flow Cytometry, Expressing, Western Blot, In Vivo, Control, Cell Counting

Journal: Journal of Gastrointestinal Oncology

Article Title: The roles and mechanisms of APOL1 in the development of colorectal cancer

doi: 10.21037/jgo-24-275

Figure Lengend Snippet: Effect of APOL1 on the biological behavior of CRC cells. (A) qRT-PCR was used to determine the APOL1 mRNA levels in HCT116, SW1116, and NCM460 cells. (B) In HCT116 cells, qRT-PCR was used to determine the interference efficacy of shAPOL1#1/2/3. (C) CCK8 assay was used to evaluate the proliferative capacity of HCT116 and SW1116 cells. (D) The count of clones in HCT116 and SW1116 cells was determined using the colony formation test. Staining method: crystal violet. Magnification: ×100. (E) Wound healing test was used to assess the migratory capacity of HCT116 and SW1116 cells (magnification: ×40). (F) The Transwell test was used to determine HCT116 and SW1116 cells’ invasion ability (magnification: ×100). Staining method: crystal violet. Each group, n=3. *, P<0.05 vs. NCM460 group; **, P<0.01; ***, P<0.001 vs . sh-NC group. sh-NC, shRNA-negative control; sh-APOL1, shRNA-apolipoprotein L1; OD, optical density; CRC, colorectal cancer; qRT-PCR, quantitative real-time polymerase chain reaction; CCK8, Cell Counting Kit 8.

Article Snippet: Shanghai iCell Bioscience Inc. (Shanghai, China) provided the human normal colon epithelial cells NCM460 (iCell-h373) and CRC cell lines [HCT116 (iCell-h071) and SW1116 cells (iCell-h201)].

Techniques: Quantitative RT-PCR, CCK-8 Assay, Clone Assay, Staining, shRNA, Negative Control, Real-time Polymerase Chain Reaction, Cell Counting

Journal: Apoptosis

Article Title: SREBP2 confers ferroptosis resistance by targeting GPX4 in colorectal cancer

doi: 10.1007/s10495-025-02209-7

Figure Lengend Snippet: SREBP2 was upregulated in human colorectal cancer. A . Real-time PCR analysis of SREBP2 expression levels in colorectal cancer and adjacent tissues. B , C . Western blotting analysis of SREBP2 in CRC tissues and adjacent tissues. The quantitative results of SREBP2 expression levels were evaluated. D , E . Immunohistochemistry of SREBP2 in tumor sections generated from CRC samples, utilizing adjacent tissues as a control. Scale bar, 50 μm. Analysis of SREBP2 IHC scores in two groups of tissue samples. F . Expression levels of SREBP2 in different CRC cell lines and normal NCM460 cells were determined by real-time PCR. G , H . Western blotting analysis of SREBP2 in different CRC cell lines and normal NCM460 cells. The quantitative results of SREBP2 expression levels were evaluated. The data are presented as the mean ± SEM of at least three independent experiments. ns, not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: The human colon epithelial cell line NCM460 and six colorectal cancer cell lines—SW480, RKO, Lovo, HT29, HCT116 and Caco2—were obtained from Procell Life Science & Technology Co (Wuhan, China).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunohistochemistry, Generated, Control